order of blood draw tubes chart
Blood Group Coombs ESR Tubes must be spun within 4 hr. When using a winged blood collection set for venepuncture a separate sodium citrate tube is drawn and DISCARDED to allow for elimination of dead space.
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Blood culture tubes Sodium citrate tubes eg blue-stopper Serum tubes with or without clot activator with or without gel separator eg red- gold- speckled-stopper Heparin tubes with or without gel eg green-stopper EDTA tubes eg lavender-stopper Glycolytic inhibitor tubes eg gray-stopper.
. Color blocks below correspond to patient bedside visual reference used at SPH following mistake proofing. Epro Item Laboratory Use Bactec Aerobic grey cap 9617 Make certain to follow the blood culture procedure Bactec Anerobic orange cap 10595 using ChloraPrep One-Step - Item 22960. 5 tubes Activated Protein C Resistance-2 tubes Factor assays 5 tubes 18 ml only please.
At 18-25 C 85mL tube or 3000 g RCF for 10 min. Order of draw chart tubes must be drawn in order of sequence 1 through 10 red black as required red section i 1 tube each light blue section ii 1 tube 1 tube each for all. Blood Culture Bottles Used for bacterial and fungal cultures.
Citrate Tube Light Blue SST TM Gel Separator Tube Red or Gold or Serum Tube Plastic or Glass - Red PST TM Gel Separator Tube Green or Heparin Tube Sodium or Lithium - Green EDTA Tube Lavender. Correct order of draw. The vacuum is set to deliver 18ml only this is the correct ratio of bloodsodium citrate solution.
By using the following mnemonic youll have an easier time remembering the proper order. Blood collection order of draw for tubes and bottles Implementation Tool Prompt Doc No. SNH0002336 v70 First Issued.
May be used for routine blood donor screening and diagnostic testing of serum for infectious disease Tube inversions ensure mixing of clot activator with blood. The recommended order is as follows. In the average adult male there are approximately 5 quarts 475 liters of blood composed of about 3 quarts 285 liters of plasma and 2 quarts 19 liters of cells.
For serum no gel tubes only. Test Tube Guide and Order of Draw Test Tube Alternate Test Tube Additive Vol. Luckily weve composed a simple chart mnemonic and infographic to easily help phlebotomists remember the proper order.
Light Green Green Gray. Blood Tube Guide and Order of Draw Reference Chart Neonatal Collection Minimum Blood Volumes. Do not freeze glass tubes.
BLOOD COLLECTION TUBES THE ORDER OF DRAW Version 82018 072010. Always follow your facilitys protocol for order of draw BD BD Logo and all other trademarks are property of Becton Dickinson and Company. The following order-of-draw is recommended when drawing several specimens during a single venipuncture and is used to avoid possible test result error due to cross contamination from tube additives.
Test Tube Chart and Order of Draw Guide Sept 2016xls Author. For clot to form keep 10 15 minutes tube at room temper-ature for minimum 30 mins. Bactec Aerobic grey cap 9617 Make certain to follow the blood culture procedure Bactec Anerobic orange cap 10595 using ChloraPrep One-Step - Item 22960 Bactec Peds pink cap 9616.
2500 g RCF for 20 min. Any blood collection sharps in biohazard containers approved for their disposal. Order of blood draw.
11122019 UNCONTROLLED WHEN DOWNLOADED Review By. At 15-25 C for further sample purification see IFU Nucleic acid PAXgeneBlood RNA tube 8-10 na na Tube guide and recommended Order of Draw1. Order Of Draw Beaumont Laboratory Tube Colors Common Laboratory Tests Phlebotomy Test Tube Medical Laboratory Science The Truth About Health Medical.
Pour off serum or plasma into a properly labeled plastic tube before freezing. Mix blood collection tubes by inverting 8 to 10 times. Blood Collection ORDER OF DRAW MIXING CHART SEPARATION Clot Spin Pour into Transport Vial Label with specimen type and patient ID For other blood serum or plasma separation.
Yellow Section III 1 tube Extra tube Green Na Hep Section IV 1 tube each Green. At 18-25 C 20mL tube Circulating cell-free DNA PAXgeneBlood ccfDNA tube 8 na 1900 g RCF for 15 min. The recommended order of draw is as follows.
Printed in USA 022002 VS5729-2 Blood Cultures 8 to 10 times Serum glass tube. Blood Cultures Sterile Tubes. Order of Draw Table Due to the many colored-top blood collection tubes it can often be difficult to remember the correct order.
All tubes must be gently inverted 8 10 times to provide thorough mixing of additives. Do not freeze whole blood unless specifically indicated by the. Blood Collection ORDER OF DRAW MIXING CHART SEPARATION Clot Spin Pour into Transport Vial Label with specimen type and patient ID For other blood serum or plasma separation instructions see the.
Codes for use at Acute Care sites using SCM Order Entry. ORDER OF DRAW CHART TUBES MUST BE DRAWN IN ORDER OF SEQUENCE 1 THROUGH 10 RED BLACK AS REQUIRED Red Section I 1 tube each Light Blue Section II 1 tube 1 tube each for all. Draw in the order shown on the chart from left to right order Label AT THE BEDSIDE Timely DELIVERY to the LAB Mix all tubes gently 5-10 times by inverting immediately after collection.
Do not exceed 60 mins. Blood cells are suspended in the plasma which is made up of water and dissolved materials including hormones antibodies and enzymes that are being carried. Order of Draw for Vacutainer Collection Tubes 1.
Draw VolumeRemarks Gold Red Gray Clot activator and gel for serum separation 5 For serum determinations in chemistry. 08032013 Page 1 of 1 Last Reviewed. 1 blood culture tube 2 coagulation tube 3 serum tube with or without clot activator or gel separator 4 heparin tube with or without gel.
Shaking the tube vigorously may cause hemolysis of the blood separating of red blood cells. Must be drawn.
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